オオニシ　ジュンジ  onishi junji
大西　淳之

  • 所属   東京家政大学  栄養学部 管理栄養学科
  •     東京家政大学大学院  人間生活学総合研究科 健康栄養学専攻
  •     東京家政大学大学院  人間生活学総合研究科 人間生活学専攻
  •     東京家政大学短期大学部  短期大学部 栄養科
  • 職種   教授
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Follicular fluid from human ovaries contains plasma kallikerin in free and in complex with α2-macroglobulin.
掲載誌名 正式名：Biomedical Research (Tokyo, Japan)
略　 称：Biomed Res
ISSNコード：03886107/1880313x
掲載区分国内
巻・号・頁 18(2),pp.161-170
著者・共著者 ◎Ohnishi J, Murata M, Kohyama K, Yoshida H, Wada S-I, Makinoda S, Fujimoto S, Takahashi T
担当区分 筆頭著者
発行年月 1997
概要 Follicular fluid from human ovaries obtained in in vitro fertilization procedures contained
substantial proteinase activities toward synthetic, arginine-containing endopeptidase
substrates. Using Pro-Phe-Arg-4-methylcoumary1-7-amide as a marker for activity, two
enzymes, designated human fluid serine proteinase-1 (hFSP-1) and -2 (hFSP-2), were
isolated. The molecular weights of intact hFSP-1 and hFSP-2 were estimated to be
approximately 90,000 and 730,000, respectively. They exhibited virtually identical
substrate specificities and inhibition profiles when tested for substrates and inhibitors
with low molecular weight. Polypeptide proteinase inhibitors, such as soybean trypsin
inhibitor and aprotinin, strongly inhibited hFSP-1, but only inhibited hFSP-2 to a limited
extent. These results, together with those of electrophoretic and immunological characterization,
revealed that hFSP-1 and hFSP-2 are free plasma kallikrein and the enzyme in complex
with the plasma inhibitor 0:2-macroglobulin, respectively. hFSP-1 activated human single-chain
precursor tissue-type plasminogen activator. This study also indicates that the
plasma kallikrein present in follicular fluid probably comes from the liver.
DOI doi: http://doi.org/10.2220/biomedres.18.161