オオニシ ジュンジ onishi junji
大西 淳之
- 所属 東京家政大学 栄養学部 管理栄養学科
- 東京家政大学大学院 人間生活学総合研究科 健康栄養学専攻
- 東京家政大学大学院 人間生活学総合研究科 人間生活学専攻
- 東京家政大学短期大学部 短期大学部 栄養科
- 職種 教授
| 論文種別 | 原著 |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | Identification of regions in the human angiotensin II receptor type I responsible for Gi and Gq coupling by mutagenesis study. |
| 掲載誌名 | 正式名：Biochemical and Biophysical Research Communications 略 称：Biochem Biophys Res Commun ISSNコード：0006291X/10902104 |
| 掲載区分 | 国外 |
| 巻・号・頁 | 218(1),pp.383-389 |
| 著者・共著者 | ◎Shibata T, Suzuki C, Ohnishi J, Murakami K, Miyazaki H. |
| 発行年月 | 1996/01 |
| 概要 | Previous mutagenesis studies of angiotensin II (Ang II) receptor type 1 (AT1) have focused on determining the regions responsible for Gq coupling using the rat AT1 receptor. We created human AT1 receptor mutants, expressed them in COS-7 cells, and identified the domains crucial for Gi coupling as well as for Gq coupling. Substitution of Asp125, Arg126, Tyr127, and Met134 by Gly, Gly, Ala, and Ala in the highly conserved sequence of the second intracellular loop in most G protein-coupled receptors provided a mutant AT1 receptor which lost the ability to couple to both Gq and Gi with no impairment in its binding to Ang II. A truncated mutant lacking the carboxyl terminal 50 residues was completely deficient in coupling to Gi, whereas it retained full ability to bind to Gq, in contrast to the rat AT1 receptor. These findings demonstrate that the cytoplasmic tail in the human AT1 receptor is the determinant of specific Gi coupling. |
| DOI | doi:10.1006/bbrc.1996.0067 |
| PMID | PMID: 8573166 |