オオニシ　ジュンジ  onishi junji
大西　淳之

  • 所属   東京家政大学  栄養学部 管理栄養学科
  •     東京家政大学大学院  人間生活学総合研究科 健康栄養学専攻
  •     東京家政大学大学院  人間生活学総合研究科 人間生活学専攻
  •     東京家政大学短期大学部  短期大学部 栄養科
  • 職種   教授
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Difference in the fate of the potent vasoconstrictors angiotensin II and endotherin internalised via their receptor-mediated endocytosis.
掲載誌名 正式名：Biomedical Research (Tokyo, Japan)
略　 称：Biomed Res
ISSNコード：03886107/1880313x
掲載区分国外
巻・号・頁 16(2),pp.97-104
著者・共著者 ◎Shibata T, Ohnishi J, Banba K, Murakami K, Miyazaki H
担当区分 2nd著者
発行年月 1995/03
概要 Receptor-mediated endocytosis of the potent vasoconstrictors, angiotensin II (All) and endothelin (ET), was studied in Chinese hamster ovary (CHO) cells stably expressing the recombinant AII receptor type lA (AT1A) and ET receptor type A (ETA), respectively. In CHO cells equilibrated with 125I-AII and 125I-ET-1 at 4°C, specifically bound radioligands were both rapidly internalized temperature-dependently with respective half times for AII and ET of 1.5 and 2.5 min at 37°C, 2 and 5 min at 30°C, 3 and 7 min at 25°C, and 8 and 24 min at 18°C. However, the fate ofinternalized ligands was strikingly different for the two peptides. Most 125I-AII was degraded and recycled back into the medium, whereas 125I-ET-l was neitherdegraded nor returned to the medium during the first 60 min after its binding to the receptor. These data suggest that the different fates of the internalized ligands might account for several unique ET-induced actions contrasting with All-elicited actions in the same target cells.
DOI doi: http://doi.org/10.2220/biomedres.16.97