シゲムラ ヤスタカ shigemura yasutaka
重村 泰毅
- 所属 東京家政大学短期大学部 短期大学部 栄養科
- 東京家政大学 家政学部 栄養学科
- 東京家政大学大学院 人間生活学総合研究科 健康栄養学専攻
- 東京家政大学大学院 人間生活学総合研究科 人間生活学専攻
- 職種 准教授
| 論文種別 | 原著 |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | Intestinal permeability of agaro-oligosaccharides: Transport across Caco-2 cell monolayers and pharmacokinetics in rats |
| 掲載誌名 | 正式名：Frontiers in Nutrition ISSNコード：2296-861X |
| 掲載区分 | 国外 |
| 巻・号・頁 | pp.996607 |
| 著者・共著者 | Ikuya Shirai, Koji Karasawa, Yusuke Kodaira, Yu Iwasaki, Yasutaka Shigemura, Hidefumi Makabe and Shigeru Katayama |
| 発行年月 | 2022/09 |
| 概要 | This study aimed to evaluate the Agaro-oligosaccharides (AOSs) permeation in the gastrointestinal tract in vivo and in vitro. Agarobiose (Abi), agarotetraose (Ate), and agarohexaose (Ahe) were detected in rat plasma after oral administration of AOSs. The detection level of agarobiose in the plasma was higher than that of agarohexaose, which was consistent with the permeation study using Caco-2 cell monolayers. Further, the adenosine triphosphate inhibitor (sodium azide) or endocytosis inhibitor (colchicine) did not inhibit AOS permeation through Caco-2 cell monolayers. Conversely, AOS permeation enhanced upon treatment with cytochalasin B, a tight junction disrupter, suggesting that AOSs might have passed mainly through the tight junctions between the intestinal epithelial cells. These results indicate that AOSs, especially agarobiose, can be absorbed as an intact form via the gastrointestinal tract across the intestinal epithelium through the paracellular pathway. |