オオニシ ジュンジ  onishi junji
大西 淳之

  • 所属   東京家政大学  家政学部 栄養学科
  •     東京家政大学大学院  人間生活学総合研究科 健康栄養学専攻
  •     東京家政大学大学院  人間生活学総合研究科 人間生活学専攻
  •     東京家政大学短期大学部  短期大学部 栄養科
  • 職種   教授
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Localization of prolyl endopeptidase mRNA in small growing follicles of porcine ovary.
掲載誌名 正式名:Molecular Reproduction and Development
略  称:Mol Reprod Dev
ISSNコード:1040452X/10982795
掲載区分国外
巻・号・頁 50(2),pp.121-127
著者・共著者 ◎Kimura A, Ohnishi J, Okimura H, Hamabata T, Takahashi T.
担当区分 2nd著者
発行年月 1998
概要 Prolyl endopeptidase (EC3.4.21.26) has been considered a unique intracellular enzyme catalyzing internal peptide bond hydrolysis of Pro-X. In this study, the distribution of prolyl endopeptidase activity and its mRNA was investigated in the follicles of porcine ovary. Both follicular fluid and granulosa cell fractions from small follicles showed higher activity than those from large follicles. Molecular cloning and Northern blot analysis suggested that only one species of prolyl endopeptidase gene was expressed in the ovary. In addition, in situ hybridization study revealed that the prolyl endopeptidase mRNA expression was more noticeable in the granulosa cell layers of small ovarian follicles than in those of large follicles, suggesting its importance in the early stage of follicular development.
PMID PMID: 9590527