オオニシ ジュンジ  onishi junji
大西 淳之

  • 所属   東京家政大学  家政学部 栄養学科
  •     東京家政大学大学院  人間生活学総合研究科 健康栄養学専攻
  •     東京家政大学大学院  人間生活学総合研究科 人間生活学専攻
  •     東京家政大学短期大学部  短期大学部 栄養科
  • 職種   教授
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Presence of follipsin complexed with α2-macroglobulin in porcine ovarian follicular fluid.
掲載誌名 正式名:Journal of experimental zoology. Part A, Comparative experimental biology
略  称:J Exp Zoolog A Comp Exp Biol
ISSNコード:15488969/1552499X
掲載区分国外
巻・号・頁 280(1),pp.57-64
著者・共著者 ◎Kohyama K, Ohnishi J, Murata M, Takahashi T.
発行年月 1998
概要 Using the follicular fluid of porcine ovaries as a source, a high-molecular-weight protein having enzyme activity toward t-butyloxycarbonyl-Gln-Arg-Arg-4-methylcoumaryl-7-amide was purified to an apparent homogeneity. Its molecular weight was estimated to be approximately 720,000. The protein was immunoprecipitated with antihuman alpha 2-macroglobulin antibody and was visualized in Western blot analysis using the same antibody. Using Western blot analysis, an 85 kDa polypeptide, which was recognized by antiporcine follipsin antibodies, was detected in this high-molecular-weight protein. When tested for substrates and inhibitors with low molecular weight, the protein showed substrate specificity and an inhibitor profile similar to those of follipsin. On the basis of these results, it was concluded that the protein is a complex of follipsin with alpha 2-macroglobulin. The complexed enzyme activated a single-chain precursor tissue-type plasminogen activator at a drastically reduced rate compared with free follipsin. The present results suggest that intrafollicular follipsin activity is regulated by the proteinase inhibitor alpha 2-macroglobulin.