オオニシ ジュンジ  onishi junji
大西 淳之

  • 所属   東京家政大学  家政学部 栄養学科
  •     東京家政大学大学院  人間生活学総合研究科 健康栄養学専攻
  •     東京家政大学大学院  人間生活学総合研究科 人間生活学専攻
  •     東京家政大学短期大学部  短期大学部 栄養科
  • 職種   教授
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 An isoform of plasma kallikrein occurring in the follicular fluid of human ovary.
掲載誌名 正式名:Biomedical Research (Tokyo, Japan)
略  称:Biomed Res
ISSNコード:03886107/1880313x
掲載区分国内
巻・号・頁 18(5),pp.369-374
著者・共著者 ◎Kudo T, Ohnishi J, Murata M, Wada S-I, Yoshida H, Fujimoto S, Takahashi T
担当区分 2nd著者
発行年月 1997
概要 Follicular fluid obtained from follicle aspirates of women participating in in vitro fertilization
program was found to contain a proteinase whose properties were very similar to those
of plasma kallikrein, but behaved differently in ion exchange column chromatographies.
The enzyme was purified to apparent homogeneity and characterized. The molecular
weight of the enzyme was estimated to be 87,000 in sodium dodecyl sulfate-polyacrylamide
gel electrophoresis, and 80,000 in gel filtration on a Sephacryl S-200 column. The
values were slightly smaller than those (90,000 in SDS-PAGE and 89,000 in the gel
filtration under the same conditions) of plasma kallikrein. The enzyme resembled plasma
kallikrein in substrate specificity, susceptibility to various proteinase inhibitors, and reactivity
to anti-human plasma kallikrein antibody. Based on these results, we tentatively
conclude that the enzyme is an isoform of plasma kallikrein.
DOI doi: http://doi.org/10.2220/biomedres.18.369