オオニシ ジュンジ  onishi junji
大西 淳之

  • 所属   東京家政大学  家政学部 栄養学科
  •     東京家政大学大学院  人間生活学総合研究科 健康栄養学専攻
  •     東京家政大学大学院  人間生活学総合研究科 人間生活学専攻
  •     東京家政大学短期大学部  短期大学部 栄養科
  • 職種   教授
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Expression of the growth hormone-releasing hormone gene and its peptide product in the rat ovary.
掲載誌名 正式名:Endocrinology
略  称:Endocrinology
ISSNコード:00137227
掲載区分国外
巻・号・頁 130(3),pp.1097-1102
著者・共著者 ◎Bagnato A, Moretti C, Ohnishi J, Frajese G, Catt KJ.
発行年月 1992
概要 GH-releasing hormone (GHRH) is a potent cAMP-mediated agonist in the rat ovary, where it binds to a common vasoactive intestinal peptide/GHRH receptor and enhances the actions of FSH on granulosa cell maturation. A GHRH-like peptide has been detected by immunocytochemistry in the human ovary and by RIA in follicular fluid, suggesting local synthesis of the peptide. In rat ovarian poly(A)+ RNA, Northern blot hybridization analysis with a 32P-labeled 48-nucleotide (nt) rat GHRH oligonucleotide probe revealed the presence of one major and two minor mRNA species. The major ovarian GHRH mRNA (1750 nt) was much larger than that present in hypothalamus and placenta (750 nt), but was similar to that observed in the rat testis. Two well defined higher mol wt forms of 3.2 and 3.6 kilobases were also present and probably represent unprocessed precursors of the 1750-nt mRNA. Further evidence of GHRH gene expression in the ovary and testis was provided by reverse transcription polymerase chain reaction of ovarian mRNA and restriction enzyme analysis of the amplified product. In addition, immunoreactive (ir) GHRH was detected in ovarian extracts and in the incubation medium of cultured rat granulosa cells. Ovaries from PMSG-treated female rats, aged 22-27 days, contained 400 +/- 25 pg/g ir-GHRH. The GHRH content of the hypothalamus of the same animals was 2.9 +/- 0.1 ng/g. Cultured rat granulosa cells released 20 +/- 0.1 pg ir-GHRH/4 x 10(5) cells.3 h into the incubation medium. The GHRH immunoreactivity detected in ovarian extracts coeluted on gel filtration chromatography with authentic rGHRH (5.2 kilodaltons). A larger form of ir-GHRH (approximately 16.5 kilodaltons) was also present.
DOI doi: http://dx.doi.org/10.1210/endo.130.3.1537276
PMID PMID: 1537276